@article {65571,
	title = {Compounds from Sedum caeruleum with antioxidant, anticholinesterase, and antibacterial activities},
	journal = {Pharm. BiolPharm. Biol},
	volume = {54},
	year = {2016},
	month = {2016},
	pages = {174{\textendash}179},
	abstract = {Context: This is the first study on the phytochemistry, antioxidant, anticholinesterase, and antibacterial activities of\&nbsp;Sedum caeruleum\&nbsp;L. (Crassulaceae). Objective: The objective of this study is to isolate the secondary metabolites and determine the antioxidant, anticholinesterase, and antibacterial activities of\&nbsp;S. caeruleum. Materials and methods: Six compounds (1{\textendash}6) were isolated from the extracts of\&nbsp;S. caeruleum\&nbsp;and elucidated using UV, 1D-, 2D-NMR, and MS techniques. Antioxidant activity was investigated using DPPH{\textbullet}, CUPRAC, and ferrous-ions chelating assays. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. Antibacterial activity was performed according to disc diffusion and minimum inhibitory concentration (MIC) methods. Results: Isolated compounds were elucidated as ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-d-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6). The butanol extract exhibited highest antioxidant activity in all tests (IC50\&nbsp;value: 28.35 {\textpm} 1.22 {\textmu}g/mL in DPPH assay, IC50\&nbsp;value: 40.83 {\textpm} 2.24 {\textmu}g/L in metal chelating activity, and IC50value: 23.52 {\textpm} 0.44 {\textmu}g/L in CUPRAC), and the highest BChE inhibitory activity (IC50\&nbsp;value: 36.89 {\textpm} 0.15 {\textmu}g/L). Moreover, the chloroform extract mildly inhibited (MIC value: 80 {\textmu}g/mL) the growth of all the tested bacterial strains. Discussion and conclusion: Ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-d-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6) were isolated from\&nbsp;Sedum caeruleum\&nbsp;for the first time. In addition, a correlation was observed between antioxidant and anticholinesterase activities of bioactive ingredients of this plant.},
	author = {Chawki Bensouici and Kabouche, Ahmed and Karioti, Anastasia and {\"O}zt{\"u}rk, Mehmet and Duru, Mehmet Emin and Bilia, Anna Rita and Kabouche, Zahia}
}
