@article {62719,
	title = {HPLC-DAD phenolics screening and in vitro investigation of haemostatic, antidiabetic, antioxidant and photoprotective properties of Centaurea tougourensis Boiss. \&amp; Reut.},
	journal = {International journal},
	volume = {67},
	number = {7},
	year = {2021},
	month = {2021},
	abstract = {
	Introduction: Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine. Objective: To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis. Methods: The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF). Results: 13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72{\textpm}0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71\%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5{\textpm}0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p\&lt;0.001) when compared to respective standards. Conclusion: This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.

},
	url = {https://herbapolonica.pl/resources/html/article/details?id=235397\&language=en},
	author = {Bensaad, Mohamed Sabri and Dassamiour, Saliha and Hambaba, Leila and Chawki Bensouici and KARIMA, OUFFROUKH and Kahoul, Mohamed Amine}
}
@article {62720,
	title = {In vitro assessment of antioxidant, anti-inflammatory, neuroprotective and antimicrobial activities of Centaurea tougourensis Boiss. \&amp; Reut.},
	journal = {Journal of Pharmacy \& Pharmacognosy Research},
	volume = {9},
	year = {2021},
	month = {2021},
	pages = {790-802},
	abstract = {
	Context: More than 500 Centaurea species compose the Asteraceae family, and most of the recent studies made on the species of this genus proved their pharmacological potential, especially to treat chronic illnesses. Aims: To evaluate for the first time the antioxidant, anti-inflammatory, neuroprotective and anti-microbial properties of the n-butanol (nBuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis. Methods: The antioxidant activity was determined by ABTS, galvinoxyl radical, phenanthroline, and reducing power assays, while the antiinflammatory effects were assessed by heat-induced hemolysis and egg albumin denaturation assays. The neuroprotective activity was assessed against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) and the anti-microbial activity by the agar disk diffusion method. Results: Both extracts possess a great antioxidant capacity, but it was considered higher in the n-BuOH extract with respective IC50 values of 8.04 {\textpm} 0.21 μg/mL in ABTS assay and 4.25 {\textpm} 0.6 μg/mL in GOR assay, while the A0.50 values were 4.46 {\textpm} 0.55 μg/mL in phenanthroline assay and 11.16 {\textpm} 0.64 μg/mL in reducing power assay. The n-BuOH extract also showed a remarkable anti-inflammatory activity with an EC50 of 120.81 {\textpm} 0.2 μg/mL in egg albumin denaturation assay and 154.15 {\textpm} 0.14 μg/mL in heat-induced hemolysis assay. The neuroprotective activity of the n-BuOH extract was very strong in both AChE and BChE inhibitory assays with respective IC50 values of 9.8 {\textpm} 0.62 μg/mL and 173.53 {\textpm} 0.04 μg/mL. EA extract was more active on microbial strains. Conclusions: These encouraging results showed once again the pharmacological potential of Centaurea species

},
	isbn = {0719-4250},
	url = {https://jppres.com/jppres/pdf/vol9/jppres21.1103_9.6.790.pdf},
	author = {Bensaad ,Mohamed-Sabri and Dassamiour, Saliha and Hambaba, Leila and Chawki Bensouici and Haba, Hamada}
}
@article {62953,
	title = {Evaluation of Cytotoxic Effect, Anti-Cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L.},
	journal = {Current Bioactive CompoundsCurrent Bioactive Compounds},
	volume = {16},
	year = {2020},
	month = {2020},
	pages = {72-79},
	isbn = {1573-4072},
	author = {Mouffouk, Chaima and Hambaba, Leila and Haba, Hamada and Mouffouk, Soumia and Chawki Bensouici}
}
@article {64803,
	title = {Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa Stellata},
	journal = {Oriental Pharmacy and Experimental MedicineOriental Pharmacy and Experimental Medicine},
	volume = {18},
	year = {2018},
	month = {2018},
	pages = {335-348},
	isbn = {2211-1069},
	author = {Mouffouk, Chaima and Hambaba, Leila and Haba, Hamada and Mouffouk, Soumia and Chawki Bensouici and Hachemi, Messaoud and Khadraoui, Hachani}
}
@article {65571,
	title = {Compounds from Sedum caeruleum with antioxidant, anticholinesterase, and antibacterial activities},
	journal = {Pharm. BiolPharm. Biol},
	volume = {54},
	year = {2016},
	month = {2016},
	pages = {174{\textendash}179},
	abstract = {Context: This is the first study on the phytochemistry, antioxidant, anticholinesterase, and antibacterial activities of\&nbsp;Sedum caeruleum\&nbsp;L. (Crassulaceae). Objective: The objective of this study is to isolate the secondary metabolites and determine the antioxidant, anticholinesterase, and antibacterial activities of\&nbsp;S. caeruleum. Materials and methods: Six compounds (1{\textendash}6) were isolated from the extracts of\&nbsp;S. caeruleum\&nbsp;and elucidated using UV, 1D-, 2D-NMR, and MS techniques. Antioxidant activity was investigated using DPPH{\textbullet}, CUPRAC, and ferrous-ions chelating assays. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. Antibacterial activity was performed according to disc diffusion and minimum inhibitory concentration (MIC) methods. Results: Isolated compounds were elucidated as ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-d-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6). The butanol extract exhibited highest antioxidant activity in all tests (IC50\&nbsp;value: 28.35 {\textpm} 1.22 {\textmu}g/mL in DPPH assay, IC50\&nbsp;value: 40.83 {\textpm} 2.24 {\textmu}g/L in metal chelating activity, and IC50value: 23.52 {\textpm} 0.44 {\textmu}g/L in CUPRAC), and the highest BChE inhibitory activity (IC50\&nbsp;value: 36.89 {\textpm} 0.15 {\textmu}g/L). Moreover, the chloroform extract mildly inhibited (MIC value: 80 {\textmu}g/mL) the growth of all the tested bacterial strains. Discussion and conclusion: Ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-d-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6) were isolated from\&nbsp;Sedum caeruleum\&nbsp;for the first time. In addition, a correlation was observed between antioxidant and anticholinesterase activities of bioactive ingredients of this plant.},
	author = {Chawki Bensouici and Kabouche, Ahmed and Karioti, Anastasia and {\"O}zt{\"u}rk, Mehmet and Duru, Mehmet Emin and Bilia, Anna Rita and Kabouche, Zahia}
}
@mastersthesis {65990,
	title = {Etude phytochimique et {\'e}valuation des activit{\'e}s biologiques de deux plantes du genre Sedum : (Crassulaceae)},
	year = {2015},
	month = {2015},
	type = {Doctorat en Sciences en Chimie Organique},
	abstract = {Les pr{\'e}sents travaux concernent deux plantes du genre Sedum (Crassulaceae). S. caeruleum et S. pubescens. L{\textquoteright}extraction et les s{\'e}parations chromatographiques (CC, CCM) des fractions chloroformique, ac{\'e}tate d{\textquoteright}{\'e}thyle et n-butanol de S. caeruleum ont permis d{\textquoteright}isoler 6 produits qui ont {\'e}t{\'e} identifi{\'e}s gr{\^a}ce aux m{\'e}thodes spectroscopiques (UV, RMN 1D et 2D)\&nbsp;: 1- Acide ursolique 2- Daucosterol (β-sitosterol-3-O-β-D-glucopyranoside) 3- β-sitosterol-3-O-β-D-galactopyranoside 4- Apig{\'e}nine 5- Apig{\'e}trine 6- Apiine L{\textquoteright}analyse LC-ESI-MS/MS de la fraction butanolique de Sedum caeruleum nous a permis d{\textquoteright}identifier 23 compos{\'e}s dont 11 acides ph{\'e}noliques et 12 flavono{\"\i}des glycosides Les r{\'e}sultats d{\textquoteright}{\'e}tude des activit{\'e}s biologiques de S. caeruleum et S. pubescens sont comme suit\&nbsp;: - Les fractions n-butanol des esp{\`e}ces S. caeruleum et S. pubescens ont montr{\'e} la meilleure activit{\'e} antiradicalaire avec le test du DPPH. - Les fractions chloroformique de S. caeruleum et butanolique de S. pubescens ont manifest{\'e} la meilleure activit{\'e} avec le test CUPRAC. - La fraction butanolique de S. caeruleum a montr{\'e} la meilleure activit{\'e} avec le test du m{\'e}tal ch{\'e}late. - Les fractions butanolique de S. caeruleum et butanolique de S. pubescens ont manifest{\'e} la meilleure activit{\'e} avec le test du β-carot{\`e}ne. - La fraction butanolique de S. caeruleum a inhib{\'e} l{\textquoteright}activit{\'e} de la butyrylcholinesterase. - La fraction chloroformique de S. caeruleum a montr{\'e} une excellente activit{\'e} antiinflammatoire sup{\'e}rieure {\`a} celle du standard (l{\textquoteright}ibuprof{\`e}ne). - La fraction chloroformique de S. caeruleum a moyennement inhib{\'e} la croissance des souches bact{\'e}riennes test{\'e}es.},
	author = {Chawki Bensouici}
}
@article {66749,
	title = {Antibacterial activity and chemical composition of the essential oil of Satureja calamintha ssp. sylvatica from Jijel, Algeria},
	journal = {Der Pharmacia LettreDer Pharmacia Lettre},
	volume = {5},
	year = {2013},
	month = {2013},
	pages = {224-227},
	abstract = {The chemical composition of the hydrodistilled essential oil of Satureja calamintha ssp. sylvatica, collected from Jijel (Eastern Algerian), was analyzed by GC and GC/MS. 23 components representing 94.45\% of the essential oil were detected with isopulegone (66.5\%) and pulegone (11.6\%) as the major components. The antibacterial activity of the essential oil was tested against 10 gram-positive and gram-negative bacteria by the use of the disc diffusion method.},
	author = {Chawki Bensouici and Benmerache, A. and Chibani, S. and Kabouche, Ahmed and Abuhamdah, S. and Z, Semra and Kabouche, Zahia}
}
@article {67035,
	title = {Analysis of the Essential Oil of Aerial Parts of Ferula luteaPoiret from Algeria},
	journal = {Journal of Essential Oil Bearing PlantsJournal of Essential Oil Bearing Plants},
	volume = {15},
	year = {2012},
	month = {2012},
	pages = {682-685},
	abstract = {The essential oil obtained by hydrodistillation of fresh aerial parts of\&nbsp;Ferula lutea\&nbsp;Poiret (Apiaceae) was analyzed by GC and GC-MS. Twenty one compounds were characterized representing 84.9 \% of the essential oil with the prevalence of 2,3,6-trimethylbenzene (25.0 \%),\&nbsp;cis-chrysanthenol (20.8 \%), α-pinene (10.9 \%) and thymol (10.2 \%).},
	author = {Chibani, Salih and Chawki Bensouici and Kabouche, Ahmed and Aburjai, Talal and Touzani, Rachid and Kabouche, Zahia}
}
@article {67034,
	title = {Flavonoids and antioxidant activity of Santolina rosmarinifolia from Algeria},
	journal = {Chemistry of Natural CompoundsChemistry of Natural Compounds},
	volume = {48},
	year = {2012},
	month = {2012},
	pages = {779{\textendash}780},
	abstract = {Santolina\&nbsp;is a small genus of the large subfamily Asteraceae of the family Compositae. Species of this genus are used in folk medicine as anthelmintic, antispasmodic, and antifectious agent [1]. Sesquiterpene lactones and volatile terpenes have been isolated from the Spanish subspecies\&nbsp;S. rosmarinifolia\&nbsp;subsp.\&nbsp;canescens, S. rosmarinifolia\&nbsp;subsp.\&nbsp;rosmarinifolia, and\&nbsp;S. chamaecyparissus\&nbsp;[2,\&nbsp;3,\&nbsp;4,\&nbsp;5]. Terpenes and acetylene derivatives were reported from the roots of\&nbsp;S. corsica\&nbsp;[6], but no flavonoids have been reported from these two subspecies. The Algerian species\&nbsp;S. rosmarinifolia\&nbsp;L. [7] has not been the subject of any phytochemical study. It will be interesting to investigate the presence of 6-methoxyflavones previously reported from\&nbsp;S. chamaecyparissus\&nbsp;[8,\&nbsp;9]. The aerial parts of\&nbsp;S. rosmarinifolia\&nbsp;were collected on may 2008 in the region of Batna (North Eastern Algerian) [7]. The voucher specimen was identified by Prof. Gerard De Belair (University Badji-Mokhtar, Annaba) and was...},
	author = {Chibani, S. and Chawki Bensouici and Kabouche, Zahia and Kabouche, Ahmed and Al-Dabbas, M.-M. and Aburjai, T.}
}
@article {67080,
	title = {Sesquiterpene lactones and flavonoids from Centaurea foucauldiana},
	journal = {Chemistry of Natural Compounds, Vol. 48, No. 3, July, 2012.Chemistry of Natural Compounds, Vol. 48, No. 3, July, 2012.},
	volume = {48},
	year = {2012},
	month = {2012},
	pages = {510{\textendash}511},
	author = {Chawki Bensouici and Kabouche, Ahmed and Kabouche, Zahia and Touzani, R. and Bruneau, C.}
}
@article {67322,
	title = {GC- MS Analysis of Ammoides atlantica (Coss. et Dur.) Wolf. from Algeria},
	journal = {Journal of essential oil - bearing plantsJournal of essential oil - bearing plants},
	volume = {14},
	year = {2011},
	month = {2011},
	pages = {481-483},
	abstract = {The essential oil of fresh aerial part of\&nbsp;Ammoides atlantica\&nbsp;Coss. et Dur. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, was analyzed by GC-MS. Twenty compounds were characterized representing 97.9 \% of the essential oil with safranal (17.9 \%),\&nbsp;endo-borneol (17.6 \%), chrysanthenone (15.5 \%), filifolone (12.1 \%) and camphor (11.8 \%) as main components.},
	author = {Boudiar, Tarek and Chawki Bensouici and Safaei-Ghomi, Javad and Kabouche, Ahmed and Kabouche, Zahia}
}
