Considering three sites under different climate conditions (arid, semi-arid, and subhumid), this study aims to use the vadose-zone water stable isotope profiles to estimate the groundwater recharge rate. High-resolution vertical subsurface soil sampling along the vadose zone of the investigated sites was conducted. The collected samples were analysed to determine their stable isotope ratios (δ²H and δ¹⁸O) that were used along with the piston displacement method to estimate recharge. Annual recharge rates of 0.2% (± 0.1%), 2.5%, and 18% of the total annual precipitation were obtained for the arid, semi-arid, and subhumid sites, respectively. Recharge rates at the semi-arid and subhumid sites are comparable to those previously estimated using water balance-based methods. The recharge rate at the arid site is lower than that previously estimated for that site using the water budget-based method, revealing difficulties in applying the piston displacement method under an arid regime.

Considering three sites under different climate conditions (arid, semi-arid, and subhumid), this study aims to use the vadose-zone water stable isotope profiles to estimate the groundwater recharge rate. High-resolution vertical subsurface soil sampling along the vadose zone of the investigated sites was conducted. The collected samples were analysed to determine their stable isotope ratios (δ²H and δ¹⁸O) that were used along with the piston displacement method to estimate recharge. Annual recharge rates of 0.2% (± 0.1%), 2.5%, and 18% of the total annual precipitation were obtained for the arid, semi-arid, and subhumid sites, respectively. Recharge rates at the semi-arid and subhumid sites are comparable to those previously estimated using water balance-based methods. The recharge rate at the arid site is lower than that previously estimated for that site using the water budget-based method, revealing difficulties in applying the piston displacement method under an arid regime.

Considering three sites under different climate conditions (arid, semi-arid, and subhumid), this study aims to use the vadose-zone water stable isotope profiles to estimate the groundwater recharge rate. High-resolution vertical subsurface soil sampling along the vadose zone of the investigated sites was conducted. The collected samples were analysed to determine their stable isotope ratios (δ²H and δ¹⁸O) that were used along with the piston displacement method to estimate recharge. Annual recharge rates of 0.2% (± 0.1%), 2.5%, and 18% of the total annual precipitation were obtained for the arid, semi-arid, and subhumid sites, respectively. Recharge rates at the semi-arid and subhumid sites are comparable to those previously estimated using water balance-based methods. The recharge rate at the arid site is lower than that previously estimated for that site using the water budget-based method, revealing difficulties in applying the piston displacement method under an arid regime.

The present study aimed to investigate the potential protective effects of zinc (Zn) against hexavalent chromium-induced hepatotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c) on the 3 rd day of pregnancy, with NaCl 0.9 % and served as control, K₂Cr₂O7 (10 mg/kg bw) alone, or K₂Cr₂O7 in association with ZnCl2 (20 mg/kg bw). Hepatic biochemical parameters, oxidative stress biomarkers and DNA damage were monitored. Results revealed that K₂Cr₂O7 disturbed plasma ALT, AST, ALP and GGT, induced hepatic oxidative stress and DNA fragmentation. The co-treatment with Zn has alleviated K2Cr2O7-induced hepatotoxicity by exhibiting antioxidant and genoprotective effects in pregnant Wistar rats.

The present study aimed to investigate the potential protective effects of zinc (Zn) against hexavalent chromium-induced hepatotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c) on the 3 rd day of pregnancy, with NaCl 0.9 % and served as control, K₂Cr₂O7 (10 mg/kg bw) alone, or K₂Cr₂O7 in association with ZnCl2 (20 mg/kg bw). Hepatic biochemical parameters, oxidative stress biomarkers and DNA damage were monitored. Results revealed that K₂Cr₂O7 disturbed plasma ALT, AST, ALP and GGT, induced hepatic oxidative stress and DNA fragmentation. The co-treatment with Zn has alleviated K2Cr2O7-induced hepatotoxicity by exhibiting antioxidant and genoprotective effects in pregnant Wistar rats.

The present study aimed to investigate the potential protective effects of zinc (Zn) against hexavalent chromium-induced hepatotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c) on the 3 rd day of pregnancy, with NaCl 0.9 % and served as control, K₂Cr₂O7 (10 mg/kg bw) alone, or K₂Cr₂O7 in association with ZnCl2 (20 mg/kg bw). Hepatic biochemical parameters, oxidative stress biomarkers and DNA damage were monitored. Results revealed that K₂Cr₂O7 disturbed plasma ALT, AST, ALP and GGT, induced hepatic oxidative stress and DNA fragmentation. The co-treatment with Zn has alleviated K2Cr2O7-induced hepatotoxicity by exhibiting antioxidant and genoprotective effects in pregnant Wistar rats.