Publications by Type: Journal Article

2024
Ghedadba N, Hambaba L, Bensaad M-S, Dassamiour S. Antihyperglycemic Potential of Methanolic Extracts from the Medicinal Plants: Marrubium deserti De Noé and Marrubium vulgare L. International Journal of Pharmacology [Internet]. 2024;207 (2) :269-278. Publisher's VersionAbstract
Background and Objective: Plants of the genusMarrubium are used to cure several infectious diseases like asthma, rheumatoid and diabetes.This study investigatedin vivo antihyperglycemic capacity of the methanolic fraction (MeOHE) of twoMarrubium species via animal model.Materials and Methods: The plant extracts were investigated both qualitatively and quantitatively to reveal their phytochemical composition.Pharmacologically speaking, Wistar albino rats of either sex (150-180 g) were used to investigate the antidiabetic effect using 25 rats in each ofthe two used approaches; respectively Oral Glucose Tolerance Test (OGTT) and streptozotocin-induced diabetes approaches. In addition, variousbiochemical parameters were investigated to confirm the pharmacological quality of our plants. Evaluated for their possible antidiabetic effectduring two consecutive weeks for glycemia levels (2 hrs period). Results: Data revealed that MeOHE induced a significant antihyperglycemic effect,in whichM. deserti, a slight decrease in the glucose level at (143±0.5 mg/dL) was obtained while a reduction was recorded forM. vulgare(71.5±0.75 mg/dL) with a same observation for triglycerides, cholesterol, glutamic-oxaloacetic transaminase (TGO), glutamic pyruvic transaminase(TGP), creatinine comparing to standard. Moreover, the result showed high levels of phenolic and flavono{\"ıds content. Conclusion: This modestwork confirmed once again the real pharmacological capacity of vegetals and their possible implication to cure diabetes and complications relatedto it underlying the antihyperglycemic activity of these plants.
Zeroual I, Benaziza A, Adjal F, Kheloufi A. Evaluation of Morphological Characteristics in Some Apple Varieties (Malus Pumila Mill.) from Northeastern Algeria. Annals of "Valahia" University of Târgovi\c ste. Agriculture [Internet]. 2024;16 (1). Publisher's VersionAbstract
The current investigation is centered on the characterization of specific apple cultivars in the Garza region, within the Teniet El Abed municipality, situated 55 km east of Batna (NE-Algeria). Utilizing descriptors outlined by UPOV 2015 pertaining to apples, the study focuses on morphological parameters of leaves, fruits, and terminal shoots across three varieties: Golden Delicious, Royal Gala, and Starkrimson. Additionally, soil analysis was conducted to ascertain its suitability for apple cultivation. The examination revealed limited morphological diversity among the investigated apple cultivars, with some descriptors, particularly those related to fruit traits, showing insignificant differences between cultivar types. Overall, the studied varieties - Golden Delicious, Royal Gala, and Starkrimson - exhibit distinct physiological and morphological characteristics. Variations in size, shape, skin color, flesh attributes, as well as sugar and acid content, contribute to the uniqueness of each variety. Whether one prefers sweet or tart apples, or favors larger or smaller fruits, there exists an apple variety to cater to individual tastes. Our findings complement molecular analyses and underscore the need for further exploration of apple germplasm, alongside sustained conservation efforts.
Kheloufi A. First record of the Checkerboard Worm Lizard, Trogonophis wiegmanni (Kaup, 1830) (Squamata: Trogonophidae) from high altitude in northeastern Algeria. Zoology in the Middle East [Internet]. 2024;70 (1). Publisher's Version
Khater N, Benahmed A, Benbellkacem I, khaoula Charouana, Benchikhelhocine I, Daas A. In vitro Propagation For the Conservation of Ilex aquifolium Threatened by the Proliferation of Axillary Shoots. African Journal of Biological Sciences [Internet]. 2024;6 (13). Publisher's VersionAbstract
Ilex aquifolium L. is an endemic species in Algeria’s Tellian and Saharan Atlas mountains in the country’s center and east, and it is on the verge of extinction owing to a variety of reasons. To help preserve it, we devised an in vitro micropropagation technique. The cuttings with axillary buds were cultured on different media supplemented with different combinations and concentrations of plant growth regulators. The best results were obtained on Woody Plants Medium (WPM) supplemented with 6-benzylaminopurine (BAP: 1.5 mg/LL, 3 mg/L) and naphthalene acetic acid (NAA: 1.5 mg/LL) in combination with BAP (1.5 mg/ L); BAP (1.5 mg/ L) with Gibberellins (GA3: 0.5 mg/ L). The best shoot proliferation rates were obtained on WPM supplemented with 1.5 mg/LL (BAP) and BAP: 1.5 mg/L; GA3: 0.5 mg/L; or BAP: 1.5 mg/L + ANA: 1.5 mg/L. The highest rooting rate (100%) was obtained with shoots grown on WPM medium supplemented with NAA (1.5 mg/LL). Absence of routing formation was observed in media containing indolacetic acid (IAA: 1 mg/ L) alone or combined with BAP: 0.5 mg/ L) The best-rooted shoots must be acclimatized to a substrate consisting of a mixture of peat and sand (1:1 v/vv), the seedlings have been acclimatized successfully.
Chedad A, Bouam I, El-Bouhissi M, Dahmani W, Ait-Hammou M, Mebarki M-T, Mezzi M, Sadine S-E. Moorish Viper, Daboia mauritanica (Gray, 1849) (Squamata, Viperidae), in Algeria: new provincial records, range extension, and an update on its distribution. Check List [Internet]. 2024;207 (2). Publisher's VersionAbstract
 Daboia mauritanica (Gray, 1849), Moorish Viper, is native to North Africa. We update the distribution map for this species in Algeria, providing 19 new locality records, filling distribution gaps and significantly expanding the species’ known range. Our updated distribution map reveals a widespread presence of the species in northern Algeria, extending south into the northern Sahara. We note that some historical records in certain provinces remain unconfirmed and/or questionable, indicating the need for further investigation. Our findings underscore the importance of collaboration between researchers and citizen scientists in Algeria.
Mekaoussi R, Benzina I, SI-BACHIR A. Morphological and Biochemical Responses of Three Durum Wheat Varieties to Aphid (Rhopalosiphum maidis) Infestation. Scientific and Technological Research Article [Internet]. 2024;25 (1). Publisher's VersionAbstract
Aphids cause biotic stress and vector diseases. However, wheat’s morphological and biochemical responses to aphid infestation are poorly studied. Our study evaluated the varietal behavior of durum wheat (Triticum durum Desf) against Rhopalosiphum maidis (Homoptera: Aphididae) in laboratory conditions. We studied morphological trait variation (plant height [HP], flag leaf area [SFDF], leaf area [SF], spike length [LE], and beard length [LB]) and biochemical characteristics (chlorophyll, soluble sugars, and proline contents) of three varieties in Algeria: MBB, BOU, and GTA. MBB is the least affected, with a 13.34 % infestation rate. The heaviest infestation occurs during the heading stage. All morphological factors decreased in length or surface between control and infested samples. ANOVA showed significant variations for HP, LE, SFDF, and SF but not LB. Biochemical parameters decrease in GTA. In MBB, chlorophyll and soluble sugars decrease while proline increases. For BOU, soluble sugars decrease while chlorophyll and proline increase. Only soluble sugars showed a significant statistical variation between control and infested plants. HP is affected by aphid infestation, which is of higher significance for MBB. Proline content is affected in BOU and GTA. There is no significant variation between aphid infestation and chlorophyll, soluble sugars, and proline levels for MBB. The effect of R. maidis infestation is less important on MBB. HP, SFDF, SF, and LE are morphological selection criteria for R. maidis infestation resistance. Proline is a biochemical selection criterion. Genetic and molecular analyses are required to identify genes for programs that enhance durum wheat’s resistance to R. maidis infestations.
Kheloufi A. On the observation of nesting of Delta dimidiatipenne (de saussure, 1852) (Hymenoptera: Vespidae: Eumeninae) from North-West Algeria. Acta Musei Silesiae, Scientiae Naturales [Internet]. 2024;73 (1). Publisher's VersionAbstract
The present study records the first observation of a female Delta dimidiatipenne constructing its nest in Oran (NW-Algeria). The nest construction and developments thereafter were monitored every three days from July to September 2022 (70 days). Ultimately four female wasps were successfully emerged. This first record will be useful for future studies on the distribution, behaviour, ecology and conservation in this semi-arid region.
Bendrihem K-A, Zeraib A, Atoki A-V, Mihoubi M-A, Kadi K, Bensuici C, Hachemi M, Ayeb N-E, Messaoudi M. Phytochemical screening, antioxidant properties, and photo-protective activities of Salvia balansae de Noé ex Coss. Open Chemistry [Internet]. 2024;22 :1-18. Publisher's VersionAbstract
The present study evaluated the potential of Salvia balansae from the Aures Mountains as a source of natural bioactive compounds. Leaves, flowers, and stems were extracted separately using methanol, ethanol, and acetone. Phenolic compounds were quantified colorimetrically and identified using liquid chromatography coupled with mass spectrometry (LC-MS). Antioxidant capacity was assessed using six different in vitro assays, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 1,1-diphenyl-1-picrylhydrazyl, cupric reducing antioxidant capacity, ferric reducing antioxidant power, ferrous ion chelating, and phenanthroline assay, while the photo-protective capacity was evaluated using the sun protective factor. The methanolic flower extracts revealed the highest O-diphenol and phenolic levels (287.9 ± 0.50 and 147.87 ± 0.21 µg GAE/mg, respectively), whereas the acetonic and ethanolic leaf extracts contained the highest flavonoid (72.17 ± 0.12 μg QE/mg) and flavonol (35.28 ± 0.05 μg QE/mg) levels, respectively. LC-MS was used to identify 18 phenolics, including quinic acid (5.051–69.69 ppm), luteolin-7-o-glucoside (7.802–44.917 ppm), apigenin-7-o-glucoside (3.751–68.507 ppm), and cirsiliol (2.081–15.608 ppm), distinguishing this Aures taxon. Principal component analysis and unweighted pair-group method with arithmetic mean revealed variability in phytochemicals, antioxidant properties, and photo-protective activity influenced by biological activities and the compound content. Overall, S. balansae demonstrated promising photo-protective capacity, the presence of key bioactive compounds, and wide-ranging antioxidant potential, presenting this endemic plant as a valuable source of natural antioxidants and photo-protective agents with pharmaceutical and cosmetic applications.
Benzina I, Bekdouche F, SI-BACHIR A. Post-fire dynamics of recolonization by Cistus plants in the Aleppo pine and Cork oak forests in Bejaia region, central north Algeria. Environmental & Socio-economic Studies [Internet]. 2024;12 (2) :40-47. Publisher's VersionAbstract
In August 2021, very intense fires swept through various forested areas in northern Algeria, particularly in Kabylia. The population dynamics of Cistus salviifolius and Cistus monspeliensis were studied along a post-fire successional gradient during the first nine months (September 2021 – May 2022), in two forest communities: the Cork oak (Quercus suber) and the Aleppo pine (Pinus halepensis) forests in the coastal zone of Bejaia province. The Cistus spp. germinations were counted monthly in each forest on 10 plots of 1 m2, evenly distributed along 2 transects of 45 m each. The results showed that the majority of germinations occur in December with 207.5 ± 24.2 seedlings in the Cork oak forest and 171.3 ± 18.0 seedlings in the Aleppo pine forest. Student’s t-test does not reveal significant differences for December between the two communities. During the first months following the fire (September and October 2021), germinations were almost absent. The monthly rainfall in September 2021 was estimated at 16.25 mm, an insufficient amount for effective germination. Late germination results in an extension of its period, showed a high rate in January 2022 with 42.2 ± 2.1 and 11.5 ± 0.8 seedlings, respectively, in the Cork oak forest and the pine forest. Beyond January, germination was practically nonexistent in both communities. Therefore, the majority of the seed germination was concentrated in November, December, and January with a unimodal germination pattern.
Mansouri L-M, Kheloufi A. Salinity effects on germination of Portulaca oleracea L.: A multipurpose halophyte from arid rangelands. Journal of Applied Research on Medicinal and Aromatic Plants [Internet]. 2024;41. Publisher's VersionAbstract
Salt resistance studies have, unfortunately, mainly been focused on conventional crops, with very few studies being conducted on the potential of available halophytes as new crops in the future. Portulaca oleracea L. has been listed as one of the most used medicinal plants by the World Health Organization as an edible halophyte. It is a fast-growing herbaceous annual with high nutritional relevance. The present work was designed to determine the effect of six soluble salts (NaCl, Na2SO4, KCl, CaCl2, CaCO3, and MgCl2) on germination of P. oleracea seeds. The effect of salinity was tested on final germination percentage (FGP%), and germination tolerance index (GTI%) using five concentrations (0, 200, 300, 400, and 600 mM) of each salt. In addition, laboratory experiments were conducted to assess the effects of salinity on germination recovery from high saline conditions (600 mM). Salinity level and salt composition significantly influenced germination attributes. P. oleracea seeds were non-dormant, exhibited approximately 100% germination in distilled water. Both FGP and GTI gradually decreased with increasing salinity. This study showed that seeds of P. oleracea can germinate under 300 mM in all tested salts. The salts causing germination inhibition exhibited specificity, with an increasing trend observed in the following sequence: MgCl2 > Na2SO4 > NaCl > KCl > CaCl2 > CaCO3. Transferring ungerminated seeds from 600 mM to distilled water recovered their germination ability. Based on these results, we can conclude that P. oleracea is a high salt-tolerant species that can tolerate a variety of salts found in soil. Therefore, P. oleracea may be considered a promising species for improving ecological balance in saline soils.

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