This paper presents a simple approach for accurate determination of the resonant frequency of an elliptical microstrip patch printed on isotropic or anisotropic substrate materials. In this approach, some modifications are made to account for fringe fields, dispersion effects, and losses by calculating effective dimensions, effective permittivity of anisotropy in the layer, and effective loss tangent, respectively. The theoretical resonant frequency results are in very good agreement with the experimental results reported elsewhere. Numerical results show that the change in the resonant frequency of the antenna is due primarily to a small disturbance of the substrate’s nature. Then the effect of the uniaxial anisotropic materials is a significant parameter and most essential on the microstrip antenna characterization.