Publications

2022
Bourarache M, Chiremsel Z, Nait-Said R. Risk Based Inspection (RBI): a performant tool fo optimizing inspection planning in Algerian process plants. 1st International Symposium on Industrial Engineering, Maintenance and Safety, March 05-06th. 2022.
Bourarache M, Chiremsel Z, Nait-Said R. Risk Based Inspection (RBI): a performant tool fo optimizing inspection planning in Algerian process plants. 1st International Symposium on Industrial Engineering, Maintenance and Safety, March 05-06th. 2022.
Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Saadali B, Zerouk H, Tarek D, Khedidja A, HAOUES C, Belloula M. Physicochemical and organic quality assessment of Mexa and Bougous waters and its suitability for agricultural use. Euro-Mediterranean Journal for Environmental Integration [Internet]. 2022;7 :79–88. Publisher's VersionAbstract

The Mexa and Bougous dams were built to control river floods and supply crop-irrigation and drinking water. This study aimed to characterize the hydrogeochemical state in the region containing the dams, which influences the quality of the waters and thus their suitability for agricultural use, given the extent of the river networks that naturally transport sediments and pollutants into the reservoirs via streams. Thus, some physicochemical and organic parameters, including electrical conductivity, calcium, magnesium, sodium, potassium, chloride, bicarbonate, sulfate, biological oxygen demand, nitrite, ammonium, and phosphate, were used as benchmarks to examine the stiffness of the water pollution. Sampling was carried out during May and September of 2011 and 2012. The obtained results showed that, according to the Stiff diagram, the dominant hydrogeochemical facies is calcium bicarbonate in waters that do not have calcium levels in excess of 3 meq/l and bicarbonate levels in excess of 2.4 meq/l. The organic pollution index disclosed that the waters have evolved from a moderate to a high degree of organic pollution due to the accumulation of pollutants and nutrients from waste disposal and fertilizers. The samples fell into the C2-S1 class in the Richards diagram; this implies that the waters are suitable for plants that are salt tolerant, but that the use of these waters can cause problems for clay soils. However, in the long term, irrigation with the dam waters may pose difficulties for agriculture due to the resulting increase in the electrical conductivity of the soil. This study concludes that water salinity and alkalinity affect crop suitability, meaning that the temporal monitoring of water quality is needed to avoid adverse consequences for crop production.

Belalite H, Menani MR, Athamena A. Calculation of water needs of the main crops and water resources available in a semi-arid climate, case of Zana-Gadaïne plain, Northeastern Algeria. Algerian Journal of Environmental Science and Technology [Internet]. 2022;8 (2). Publisher's VersionAbstract

The relative scarcity of water resources in Algeria and their unequal distribution induce a rational use of available resources. The Zana-Gadaïne plain appears as an exemplary case study, where the difficulties posed by the problem of crop water needs versus the availability of water resources appear. This article, based on field surveys and in-situ measurements, aims to identify the pressure of irrigation on water resources and the optimization of their use in an agricultural area, where irrigated agriculture represents 85% of the water consumption of the Zana-Gadaïne plain. The piezometric study in correlation with hydrogeological data reveals that groundwater resources are limited, aggravated by wastage resulting in a consequent drawdown of 24 meters over 11 years. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops.

Belalite H, Menani MR, Athamena A. Calculation of water needs of the main crops and water resources available in a semi-arid climate, case of Zana-Gadaïne plain, Northeastern Algeria. Algerian Journal of Environmental Science and Technology [Internet]. 2022;8 (2). Publisher's VersionAbstract

The relative scarcity of water resources in Algeria and their unequal distribution induce a rational use of available resources. The Zana-Gadaïne plain appears as an exemplary case study, where the difficulties posed by the problem of crop water needs versus the availability of water resources appear. This article, based on field surveys and in-situ measurements, aims to identify the pressure of irrigation on water resources and the optimization of their use in an agricultural area, where irrigated agriculture represents 85% of the water consumption of the Zana-Gadaïne plain. The piezometric study in correlation with hydrogeological data reveals that groundwater resources are limited, aggravated by wastage resulting in a consequent drawdown of 24 meters over 11 years. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops.

Belalite H, Menani MR, Athamena A. Calculation of water needs of the main crops and water resources available in a semi-arid climate, case of Zana-Gadaïne plain, Northeastern Algeria. Algerian Journal of Environmental Science and Technology [Internet]. 2022;8 (2). Publisher's VersionAbstract

The relative scarcity of water resources in Algeria and their unequal distribution induce a rational use of available resources. The Zana-Gadaïne plain appears as an exemplary case study, where the difficulties posed by the problem of crop water needs versus the availability of water resources appear. This article, based on field surveys and in-situ measurements, aims to identify the pressure of irrigation on water resources and the optimization of their use in an agricultural area, where irrigated agriculture represents 85% of the water consumption of the Zana-Gadaïne plain. The piezometric study in correlation with hydrogeological data reveals that groundwater resources are limited, aggravated by wastage resulting in a consequent drawdown of 24 meters over 11 years. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops. The analysis of interannual climate variability has enabled us to draw rainfall maps characteristic of the evolution of rainfall over the past decades where we observe a net deficit in precipitation. We calculated the evapotranspiration and the requirements in irrigation water for each crop in order to compare them with the available hydric resources and the establishment of irrigation schedules for the principal irrigated crops.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

HANFER M, Benramdane Z, Cheriet T, Sarri D, Menad A, Mancini I, Seghiri R, Ameddah S. Chemical constituents, in vitro anti-inflammatory, antioxidant and hemostatic activities of then-butanol extract ofHyacinthoides lingulata (Poir.) Rothm. Natural Product Research [Internet]. 2022. Publisher's VersionAbstract

The phytochemical profile obtained from LC-ESI-MS/MS analysis of then-butanol extract (BEHL) from the North African endemic plantHyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract,in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH andβ-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

Loucif L, Chelaghma W, Cherak Z, Bendjama E, Beroual F, Rolain J-M. Detection of NDM-5 and MCR-1 antibiotic resistance encoding genes in Enterobacterales in long-distance migratory bird species Ciconia ciconia, Algeria. Science of The Total Environment [Internet]. 2022;814. Publisher's VersionAbstract

β-lactams and colistin resistance in Enterobacterales is a global public health issue. In this study we aimed to investigate the occurrence and genetic determinants of Extended-Spectrum β-lactamases, carbapenemases and mcr-encoding-genes in Enterobacterales isolates recovered from the migratory bird species Ciconia ciconia in an Algerian city. A total of 62 faecal samples from white storks were collected. Samples were then subjected to selective isolation of β-lactams and colistin-resistant-Enterobacterales. The representative colonies were identified using Matrix-Assisted Laser Desorption-Ionisation Time-of-Flight Mass Spectrometry. Susceptibility testing was performed using the disk-diffusion method. ESBL, carbapenemases, and colistin resistance determinants were searched for by PCR and sequencing. The clonality relationships of the obtained isolates were investigated by multilocus sequence typing assays. Mating experiments were carried out to evaluate the transferability of the carbapenemase and mcr-genes. Forty-two isolates were identified as follows: Escherichia coli (n = 33), Klebsiella pneumoniae (n = 4), Proteus mirabilis (n = 4) and Citrobacter freundii (n = 1). Molecular analysis showed that twelve isolates carried the blaESBL genes alone, fifteen E. coli isolates were positive for the blaOXA-48 gene, six isolates were NDM-5-carriers (two P. mirabilis, two K. pneumoniae and two E. coli) and eight E. coli strains were positive for the mcr-1 gene. MLST results showed a high clonal diversity, where NDM-5-producing strains were assigned to two sequence types (ST167 for E. coli and ST198 for K. pneumoniae), whereas the mcr-1 positive E. coli isolates belonged to ST58, ST224, ST453, ST1286, ST2973, ST5542, ST9815 and the international high-risk resistant lineage ST101. To the best of our knowledge, this is the first report of blaNDM-5 gene in white storks and also the first describing the mcr-1 gene in white storks in Algeria. This study underlines the important role of migratory white storks as carriers of high-level drug-resistant bacteria, allowing their possible implication as indicators and sentinels for antimicrobial resistance surveillance.

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